Fig. 6: Replication elongation factors suppress Rad52-dependent SSA at centromeres.

a Illustration of ade6B and ade6X integrated at the SnaBI sites in cen1. Sn; SnaBI. b rad54∆ and pol1-R961K rad54∆ cells (TNF3983 and its derivative) were grown on non-selective (n/s) EMM + AL media and replicated on EMM + LG plates, on which only Ade⁺ recombinants grow. The cells were grown at 33 °C. c The pol1-R961K mutation in the catalytic domain of Pol1. d Gene conversion rates at cen1 in the wild-type, rad51∆, rad54∆, rad52∆, pol1-R961K, rad51∆ pol1-R961K, rad54∆ pol1-R961K, and rad52∆ pol1-R961K strains (TNF3347, 3446, 3452, 3459, 4235, 4300, 4252, and 4253, respectively). e Pie charts showing the proportions of Rad51-dependent recombination and (Rad51-independent but) Rad52-dependent recombination at cen1 in wild-type and pol1-R961K cells. f Gene conversion rates at cen1 in the wild-type, rad52-R45K, pol1-R961K, rad52-R45K pol1-R961K, rad51∆, rad51∆ rad52-R45K, rad51∆ pol1-R961K, and rad51∆ rad52-R45K pol1-R961K strains (TNF3347, 5999, 4235, 6009, 3446, 6019, 4300, and 6037, respectively). g Gene conversion rates at ura4 in the wild-type, rad51∆, rad54∆, rad52∆, pol1-R961K, rad51∆ pol1-R961K, rad54∆ pol1-R961K, and rad52∆ pol1-R961K strains (TNF3631, 3635, 3645, 3643, 4215, 4371, 4378, and 4350, respectively). h Pie charts showing the proportions of Rad51-dependent recombination and (Rad51-independent but) Rad52-dependent recombination at ura4. i Gene conversion rates at cen1 in the wild-type, rad51∆, rad52∆, swi6∆, rad51∆ swi6∆, rad52∆ swi6∆, cdc18-K46, rad51∆ cdc18-K46, rad52∆ cdc18-K46, cdc20-M10, rad51∆ cdc20-M10, rad52∆ cdc20-M10, swi1∆, rad51∆ swi1∆, and rad52∆ swi1∆ strains (TNF3347, 3446, 3459, 3710, 4542, 6655, 5096, 5155, 6632, 4594, 4617, 5037, 5018, 5033, and 6653, respectively). j Illustration of the protein components of the replication machinery. *P < 0.05; **P < 0.01; ****P < 0.0001; ns, non-significant P > 0.05. Source data for the graphs in d–i are available in Supplementary Data 1.