Fig. 1: Isolated adult murine cardiomyocytes (aCMs) cultured on Geltrex and treated with blebbistatin show sustained viability and retention of functional protein expression patterns up to 7 days post-isolation. | Communications Biology

Fig. 1: Isolated adult murine cardiomyocytes (aCMs) cultured on Geltrex and treated with blebbistatin show sustained viability and retention of functional protein expression patterns up to 7 days post-isolation.

From: Functional culture and in vitro genetic and small-molecule manipulation of adult mouse cardiomyocytes

Fig. 1: Isolated adult murine cardiomyocytes (aCMs) cultured on Geltrex and treated with blebbistatin show sustained viability and retention of functional protein expression patterns up to 7 days post-isolation.

a Confocal analysis of aCMs of α-actinin and RyR2, DHPRα, SERCA2a, and STIM1 (green) up to one week in culture. Nuclei were visualized with Hoechst 33342 (blue). Scale bars equal 20 µm. b Immunoblots for Akt, pAkt, and α-tubulin of aCM lysates after 24 h culture on Geltrex-coated surfaces (N = 3, p = 0.023). c Cell counting revealed the survival of viable rod-shaped (red) relative to rounded aCMs (blue) (N = 3). All data expressed as mean ± SEM; N denotes biological replicates; significance indicated by *(p < 0.05).

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