Fig. 2: Adult cardiomyocyte cultures using a combination of Geltrex or laminin and the contractile inhibitor blebbistatin or butanedione monoxime over 1 week-post isolation. | Communications Biology

Fig. 2: Adult cardiomyocyte cultures using a combination of Geltrex or laminin and the contractile inhibitor blebbistatin or butanedione monoxime over 1 week-post isolation.

From: Functional culture and in vitro genetic and small-molecule manipulation of adult mouse cardiomyocytes

Fig. 2: Adult cardiomyocyte cultures using a combination of Geltrex or laminin and the contractile inhibitor blebbistatin or butanedione monoxime over 1 week-post isolation.

a The combination of Geltrex (GT; solid lines) and blebbistatin (Blebbi; hot colors) produced a high ratio of rod-shaped myocytes (squares) to rounded (circles) cells compared to other component combinations using more conventional culture conditions including butanedione monoxime (BDM; cool colors) or laminin-521 (Lam; dotted lines). b Confocal imaging of DHPRα expression after 7 days in isolation. Striations and fewer puncta were seen in cultures with GT and blebbistatin than other combinations; scale bars represent 20 µm. All data expressed as mean ± SEM; N = 3 biological replicates except for the GT + BDM group (N = 4) and significant differences from the GT + blebbistatin culture treatment are indicated by *(p < 0.05), **(p < 0.01) and ***(p < 0.001) for both main effects and individual timepoints.

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