Table 1 Cryo-EM data collection, refinement, and validation statistics.
HsLRRC8D (EMD-30029) (PDB 6M04) | |
|---|---|
Data collection and processing | |
Magnificationa | x23,500 |
Voltage (kV) | 200 |
Electron exposure (e–/Å2) | 50 |
Defocus range (μm) | 0.5–2.5 |
Pixel size (Å) | 1.490 |
Symmetry imposed | C2 |
Initial particle images (no.) | 2,056,807 |
Final particle images (no.) | 247,154 |
Map resolution (Å) | 0.143 |
FSC threshold | |
Map resolution range (Å) | 3.9–6.0 |
Refinement | |
Initial model used (PDB code) | 5ZSU, 6G9O |
Model resolution (Å) | 0.5 |
FSC threshold | |
Model resolution range (Å) | 4.36 |
Map sharpening B factor (Å2) | −211.2 |
Model composition | |
Non-hydrogen atoms | 34,666 |
Protein residues | 4282 |
B factors (Å2) | |
Protein | 106.9 |
R.m.s. deviations | |
Bond lengths (Å) | 0.007 |
Bond angles (°) | 1.288 |
Validation | |
MolProbity score | 2.38 |
Clashscore | 10.11 |
Poor rotamers (%) | 2.06 |
Ramachandran plot | |
Favored (%) | 87.11 |
Allowed (%) | 12.85 |
Disallowed (%) | 0.05 |
