Fig. 3: Transcriptomics shows fat-depot-specific response to influenza.

SCAT and EWAT from mock-treated and IAV-infected mice were treated for transcriptomic analysis at 7 dpi, n = 4 mock-treated animals and n = 4 IAV-infected animals. a Venn diagrams summarizing the number of distinct and overlapping differentially expressed genes (DEGs) in the SCAT and EWAT from IAV-infected mice vs. the SCAT and EWAT from mock-treated mice. Upregulated (UR) and downregulated (DR) DEGs numbers are shown. b The top five canonical pathways identified by Ingenuity Pathway Analysis (IPA) for the genes upregulated in SCAT and EWAT upon infection (IFN = interferon, IRF = interferon regulatory factor, RA = retinoic acid). The corresponding upstream regulators and their target molecules are shown in Supplementary Table 1. c The top five canonical pathways identified by IPA for the genes downregulated in SCAT and EWAT upon infection (SP = superpathway). The corresponding upstream regulators and their target molecules are shown in Supplementary Table 3. d The top five canonical pathways identified by IPA for the genes downregulated upon infection exclusively in SCAT (TCA cycle = tricarboxylic acid cycle). For (b) to (d), pathways were sorted by p values and absolute z-scores. Intensities of red indicate the higher or lower value of positive z-scores (activated pathways), intensities of blue indicate the higher or lower value of negative z-scores (inhibited pathways), gray indicates pathways having no activity pattern available since no z-score could be calculated. The spotted line indicates IPA’s default threshold.