Fig. 7: Influenza induces metabolic reprogramming of preadipocytes.

Murine preadipocytes (3T3-L1 cells) and adipocytes (differentiated from 3T3-L1 cells) were infected at a MOI of 1, and samples were prepared for transcriptomic analysis at 24 hpi, n = 4 biologically independent samples per condition. a Venn diagrams showing the numbers and intersections of the differentially expressed genes (DEGs), either upregulated or downregulated, in IAV-infected vs. mock-treated preadipocytes and adipocytes in the whole-genome expression profiling microarray data set. Top canonical pathways and related upstream regulators are listed in Supplementary Table 4 (genes commonly upregulated upon infection in preadipocytes and adipocytes), Supplementary Table 5 (genes only upregulated upon infection in adipocytes), Supplementary Table 6 (genes only upregulated upon infection in preadipocytes), and Supplementary Table 7 (genes only downregulated upon infection in preadipocytes). b Scheme of the serine-glycine-one-carbon (SGOC) metabolic pathway. Genes coding for cytosolic and mitochondrial SGOC enzymes (in red) that were upregulated in preadipocytes after IAV infection are framed. c Relative mRNA expression (RT-qPCR) of genes related to ribosome biogenesis (Urb2, Wdr75, Zfp593, Mrto4, and Nop58) in mock-treated and IAV-infected preadipocytes and adipocytes (MOI of 1, 24 hpi), n = 12 biologically independent samples per condition. Data are expressed as mean ± SD. *p < 0.05, ***p < 0.001, ****p < 0.0001. Differences between mock-treated and IAV-infected groups (c) were considered significant when p < 0.05.