Fig. 1: WIP transcription factors in Arabidopsis and Melon.
a Phylogenetic tree with the six WIP genes from Arabidopsis and melon. The closest homologue of CmWIP1 in Arabidopsis is NTT, involved in transmitting tract development in Arabidopsis. NTT together with WIP4 and WIP5 are required for initiation of the root meristem. b Rosettes and leaves of WT Col-0 and 35S:CmWIP1 transgenic lines, 35S:CmWIP1 shown serrated leaves, as previously described in 35S:TT1 and 35S:NTT8,13. Scale bar = 0.5 cm. c Vanillin staining of Col-0, tt1 mutant, and transgenic immature seeds using TT1 and CmWIP1 under the control of TT1 promoter (pTT1) to complement yellow seeds phenotype of tt1 mutants. Scale bar = 20 µm. d Quantification of pro-anthocyanidins (PA) in Col-0, tt1 mutant, and transgenic mature seeds. Bars indicate standard errors of two biological repeats and three technical repeats. Asterisks show significant differences compared to tt1 mutant (Student t-test, p < 0.05, n = 6 biologically independent samples, each corresponding to a pool of 10 mg mature seeds).
