Fig. 1: Expanded-host range of Guarani isolated from Tupanvirus cocultures. | Communications Biology

Fig. 1: Expanded-host range of Guarani isolated from Tupanvirus cocultures.

From: A virophage cross-species infection through mutant selection represses giant virus propagation, promoting host cell survival

Fig. 1

a, b Histograms depicting the genome replication, during primo-cocultures, of Guarani, Zamilon and Sputnik in Acanthamoeba castellanii coinfected with Tupanviruses. a Replication of virophages with Tupanvirus Deep Ocean. b Replication of virophages with Tupanvirus Soda Lake. c, d Graphs depicting genome replication of Guarani isolated from Tupanvirus supernatants in Acanthamoeba castellanii coinfected with each Tupanvirus strain. c Guarani isolated from Tupanvirus Deep Ocean supernatant. d Guarani isolated from Tupanvirus Soda Lake supernatant. The DNA replication of each virophage at times 0, 24, and 48 h p.i. was measured by quantitative real-time PCR (for (c) and (d), only times 0 and 48 p.i. are shown here). The increase in the amount of virophage DNA (fold of induction) was then calculated using the delta Ct method considering the difference between the Ct values specific to each virophage at times H0 and H48. Amoebas infected only with each Tupanvirus was used as negative control. All the PCRs targeting these controls were negatives at times H0, H24, and H48. Error bars, standard deviation (n = 3 biologically independent experiments). N.M.: No multiplication.

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