Fig. 1: The expression of UBE2K decreases during differentiation. | Communications Biology

Fig. 1: The expression of UBE2K decreases during differentiation.

From: The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells

Fig. 1

a Western blot analysis with antibody to UBE2K. The graph represents the UBE2K relative percentage values (corrected for β-actin loading control) to H9 hESCs (mean ± s.e.m. of three independent experiments). b Quantitative PCR (qPCR) analysis of UBE2K mRNA levels. Graph (relative expression to H9 hESCs) represents the mean ± s.e.m. of three biologically independent samples. c, d Western blot analysis with antibody to UBE2K. The graphs represent the UBE2K relative percentage values (corrected for β-actin) to H9 hESCs (mean ± s.e.m. of three independent experiments). e Western blot analysis of UBE2K levels comparing iPSCs with their parental HFF-1 fibroblasts. The graph represents the UBE2K relative percentage values (corrected for β-actin) to HFF-1 fibroblasts (mean ± s.e.m. of three independent experiments). f UBE2K mRNA relative levels to HFF1 fibroblasts represent the mean ± s.e.m. (n = 6 biologically independent samples). All the statistical comparisons were made by two-tailed Student’s t-test for unpaired samples. P value: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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