Fig. 6: NPCs derived from UBE2K shRNA hESCs retain alterations in both total H3 levels and H3K9me3/H3 ratio. | Communications Biology

Fig. 6: NPCs derived from UBE2K shRNA hESCs retain alterations in both total H3 levels and H3K9me3/H3 ratio.

From: The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells

Fig. 6

a After 10 days of neural induction treatment, early H9 NPCs were analysed by western blot with antibodies to total H3 and H3K9me3. Graphs represent the histone H3/β-actin and H3K9me3/H3 relative percentage values to NT shRNA NPCs (mean ± s.e.m., three independent experiments). b After 10 days of neural induction, early H9 NPCs were assessed by immunofluorescence with H3K9me3 and Hoechst staining. Scale bar represents 20 μm. All the statistical comparisons were made by two-tailed Student’s t test for unpaired samples. P value: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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