Fig. 8: Ectopic expression of UBE2K in UBE2K-deficient hESCs rescues H3K9me3 levels and induction of neuronal genes. | Communications Biology

Fig. 8: Ectopic expression of UBE2K in UBE2K-deficient hESCs rescues H3K9me3 levels and induction of neuronal genes.

From: The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells

Fig. 8

a Western blot analysis of H9 hESCs with antibodies to H3K9me3, total H3, UBE2K and β-actin. The images are representative of two independent experiments. b, c qPCR analysis after 20 days of neural induction of H9 hESCs. Graph (relative expression to NT shRNA) represents the mean ± s.e.m. of four independent experiments. All the statistical comparisons were made by two-tailed Student’s t test for unpaired samples. P value: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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