Fig. 4: Analysis and quantification of Δψm and mitochondrial ROS. | Communications Biology

Fig. 4: Analysis and quantification of Δψm and mitochondrial ROS.

From: High-content fluorescence imaging with the metabolic flux assay reveals insights into mitochondrial properties and functions

Fig. 4

a TMRE fluorescence plotted with respect to OCR in T3M4 cells. b OCR readout following treatment across a dose range of FCCP in T3M4 cells. n = 8 biologically independent samples. c Distribution analysis of TMRE fluorescence intensity (A.U.) in T3M4 cells. n = 50 biologically independent samples per bin. d Exponential curve fit of OCR data of PA-TU-8902 pancreatic cancer cells treated with either DTBP (ditertbutyl peroxide; red lines) or hydrogen peroxide (H2O2; blue lines). n = 5 biologically independent samples. e Violin plots displaying induction of MitoSOX fluorescence (A.U.) following either DTBP or H2O2 treatment. n = 5 to 10 biologically independent samples. f Multi-analysis plot displaying change in OCR values plotted against MitoSOX fluorescence in PA-TU-8902 cells post DTBP or H2O2 treatment. n = 5 biologically independent samples. All experiments were the result of ≥2 independent experiments. All measurements were obtained from distinct samples.

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