Fig. 4: Focused refinement of ACP stalled at the DH domain. | Communications Biology

Fig. 4: Focused refinement of ACP stalled at the DH domain.

From: Steric occlusion regulates proximal interactions of acyl carrier protein domain in fungal fatty acid synthase

Fig. 4: Focused refinement of ACP stalled at the DH domain.The alternative text for this image may have been generated using AI.

a Focused region and signal subtracted region are shown in gray and transparent light gray, respectively. Two slices of the unsharpened map are shown to the left. b Atomic models (PDB: 2UV8) flexibly fitted to sharpened map. Distances between catalytic residues5 of DH (Cδ of D1559 and Nɛ of H1564) and ER (Nɛ of H740) domains to the Cβ of S180 of ACP are given in parenthesis. c Focused 3D classification showing domain mobility of ACP stalled at DH. Particle distribution was ~50% for both 3D classes. Both maps are shown at the same threshold and low-pass filtered to 10 Å.

Back to article page