Fig. 4: TA and DOP did not inhibit the HaCaT cell proliferation and the increase of intracellular Ca++.
From: Trace amines produced by skin bacteria accelerate wound healing in mice
a HaCaT cells proliferations were measured using MTT assay. The presence of EPI (25 µg/ml) increased the cell proliferations compared to control. The addition of TAs and DOP (25 µg/ml) showed no significant effect. b The intracellular Ca++ was measured using fluorescence-based assay. HaCaT cells was incubated in the presence of EPI (25 µg/ml) alone, and EPI with TA, DOP, and ICI. EPI increased the intracellular Ca++ level with and without TA and DOP at 3 min ICI nullified the effect of EPI both in terms of cells proliferation and increase in intracellular Ca++. For all graphs, each data point is the mean value ± SEM from three independent replicates, *p < 0.05; **p < 0.01; and ***p < 0.001, data were analyzed using Student's t test. Source data are provided as a Supplementary Data 1 file.
