Fig. 6: Cellular and morphological evaluation of pre-innervated bioscaffolds following implantation in a VML model. | Communications Biology

Fig. 6: Cellular and morphological evaluation of pre-innervated bioscaffolds following implantation in a VML model.

From: Pre-innervated tissue-engineered muscle promotes a pro-regenerative microenvironment following volumetric muscle loss

Fig. 6

a–h Longitudinal sections near the repair site of animals implanted with nanofibers with motor neurons + myocytes (MN-MYO) at1 week (a–d) and 3 weeks (e–h) time point. The nanofiber sheets were coated with Laminin prior to culturing cells and hence the stacked sheets were identified based on Laminin stain (red). Scale bar = 500 µm. (a′–h′) Magnified view of the corresponding region inside the white box. Thick bundles of myocytes (Phalloidin: green) and motor axons (NF: purple) were observed within the stacked nanofiber sheets both at 1 week and 3 weeks’ time point. Scale bar = 100 µm.

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