Fig. 5: FXR activation attenuated osteogenic medium-induced HAMSCs inflammation and calcification via increasing miR-135a-5p. | Communications Biology

Fig. 5: FXR activation attenuated osteogenic medium-induced HAMSCs inflammation and calcification via increasing miR-135a-5p.

From: Farnesoid X receptor activation inhibits TGFBR1/TAK1-mediated vascular inflammation and calcification via miR-135a-5p

Fig. 5

a TGF-β pathway-associated microRNAs differentially expressed in osteogenic medium-cultured HASMCs assayed by high-throughput sequencing. b The expression of microRNA in osteogenic medium-cultured HASMCs in the absence or presence of OCA. c MiR-135a-5p expression of aortas in indicated groups (n = 8 per group). d MiR-135a-5p expression of HASMCs in indicated groups. e Intercellular NF-κB content of HASMCs in indicated groups. f Western blot analysis of NF-κB and TNF-α expression in HASMCs in indicated groups. g Calcium content of HASMCs in indicated groups. h Representative images of alizarin red staining in indicated groups. *P < 0.05, **P < 0.01, ns indicates not significant. Data were pooled as mean ± S.D. (error bars) from three or more independent experiments.

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