Fig. 3: Global analysis of P. falciparum multigene expression patterns.
From: Rapid activation of distinct members of multigene families in Plasmodium spp
a–c Transcriptomic analysis of var (a), stevor (b) and rifin (c) expression after knock-in of one var (Pf3D7_0421300), one stevor (Pf3D7_1149900) or one rifin (Pf3D7_1254800) and compared to parental 3D7 across 3 timepoints (18, 24 and 30 hpi). Blue indicates downregulation, yellow upregulation as compared to refence pool RNA. Data are given as log fold change. Only genes which are either 1.5fold up or downregulated compared to the pool and are detected in at least six samples are shown. Black arrows mark the targeted gene. a, b Forcing expression of a specific stevor or var leads to upregulation of that particular transcript and downregulation of all other members. Only one highly expressed var gene could be detected after stevor knock-in. c Forcing expression of a particular rifin does not have an impact on overall rifin expression. d qRT-PCR validation of microarray results. RNA was harvested 6 weeks after the microarray analysis and grown in the blood of three different donors. V, r and s behind the geneID indicate var, rifin or stevor multigene family, respectively. Overall expression pattern remained constant. All data are presented as mean ± standard error of the mean (SEM).
