Fig. 5: Studies of elamipretide interactions with CL. | Communications Biology

Fig. 5: Studies of elamipretide interactions with CL.

From: The cardiolipin-binding peptide elamipretide mitigates fragmentation of cristae networks following cardiac ischemia reperfusion in rats

Fig. 5

Lipidomics indicated declines in total (a) and tetra-linoleoyl CL (b) after ischemia-reperfusion. Biomimetic models of the mitochondrial inner membrane lipids presented in c–f. d GUV imaging of mitochondrial CL (NAO) and fluorescent TAMRA-elamipretide indicate elamipretide-mediated CL and vesicle aggregation (N = 3 experiments, 40–100 images per group). c Mean molecular area at physiological membrane pressure (30 mN/m) indicates consequences of losing CL content (N = 3–6 per group). Elamipretide augmented mean molecular area despite CL losses (modeled after the mass spectrometry data in panel a. More severe (50%) declines in total CL were also modeled. d Biomimetic membrane studies also determined effects of CL peroxidation and effects of elamipretide. f Proposed model in which elamipretide aggregates CL headgroups to increase mean molecular area. *P < 0.05 v control, #P < 0.05 v. I/R.

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