Table 1 Figures of merit for the standard cell classification.

From: Rapid detection of microbiota cell type diversity using machine-learned classification of flow cytometry data

Standard

  

Percentage (Mean ± st dev)a

abbreviation

Full name

remark

Recallb

Precisionb

Correct predicted classification in LWc

B02

Beads 0.2 µm

 

98.3 ± 0.1

99.0 ± 0.1

98.5 ± 0.5

B05

Beads 0.5 µm

 

99.4 ± 0.1

98.7 ± 0.1

99.3 ± 0.5

B1

Beads 1 µm

 

99.8 ± 0.1

99.6 ± 0.1

99.8 ± 0.3

B2

Beads 2 µm

 

99.4 ± 0.2

99.6 ± 0.2

99.3 ± 0.3

B4

Beads 4 µm

 

96.3 ± 0.3

97.8 ± 0.5

96.3 ± 0.5

B6

Beads 6 µm

 

98.1 ± 0.4

96.5 ± 0.3

97.6 ± 0.6

B10

Beads 10 µm

 

98.8 ± 0.3

99.8 ± 0.1

99.1 ± 0.3

B15

Beads 15 µm

 

99.8 ± 0.0

98.9 ± 0.3

99.7 ± 0.2

AJH1

Acinetobacter johnsonii

subpop 1

88.7 ± 0.6

79.2 ± 0.5

88.5 ± 1.7

AJH2

 

subpop 2

90.9 ± 1.0

77.9 ± 0.8

90.7 ± 2.0

ATJ1

Acinetobacter tjernbergiae

subpop 1

56.2 ± 2.1

47.0 ± 1.0

57.9 ± 0.9

ATJ2

 

subpop 2

58.8 ± 3.8

59.5 ± 2.7

59.1 ± 0.5

ACH1

Arthrobacter chlorophenolicus

subpop 1

72.7 ± 1.1

56.8 ± 0.8

74.8 ± 0.7

ACH2

 

subpop 2

63.4 ± 1.3

66.1 ± 1.9

63.5 ± 2.4

ACH3

 

subpop 3

78.2 ± 0.9

70.6 ± 2.4

74.1 ± 3.2

BST1

Bacillus subtilis

subpop 1

97.8 ± 0.3

95.7 ± 4.3

92.9 ± 0.2

BST2

 

subpop 2

80.8 ± 0.9

76.6 ± 1.1

81.2 ± 1.4

CCR1

Caulobacter crescentus

subpop 1

54.0 ± 2.0

62.0 ± 1.9

53.2 ± 0.1

CCR2

 

subpop 2

79.5 ± 1.9

83.1 ± 1.2

78.3 ± 4.7

CAL

Cryptococcus albidus

 

99.9 ± 0.0

99.8 ± 0.1

99.8 ± 2.0

MG_EXP3

Escherichia coli MG1655

exponential phase

88.2 ± 0.6

87.5 ± 1.1

87.8 ± 0.5

MG_STAT_LB

 

stationary phase LB

89.3 ± 1.0

90.0 ± 0.7

88.7 ± 1.3

MG_STAT_MM

 

stat phase M9-CAA

97.4 ± 0.8

96.7 ± 0.8

97.7 ± 1.8

DH_STAT_LB

Escherichia coli DH5α-λpir

 

73.0 ± 0.9

83.5 ± 1.1

72.6 ± 0.6

LLC

Lactococcus lactis

 

34.0 ± 3.3

49.9 ± 3.0

34.8 ± 1.8

PKM1

Pseudomonas knackmussii

 

94.0 ± 0.8

87.7 ± 0.7

93.6 ± 1.1

PMG

Pseudomonas migulae

 

32.9 ± 2.8

39.5 ± 3.9

32.6 ± 3.1

PPT

Pseudomonas putida

 

27.3 ± 4.0

38.2 ± 3.4

27.5 ± 3.5

PVR1

Pseudomonas veronii

subpop 1

73.9 ± 0.7

77.2 ± 1.9

74.3 ± 4.9

PVR2

 

subpop 2

96.7 ± 0.7

96.7 ± 0.7

96.8 ± 0.7

SWT

Sphingomonas wittichii

 

44.1 ± 1.5

52.7 ± 3.4

44.4 ± 1.5

SYN

Sphingomonas yanoikuyae

 

66.6 ± 1.2

55.6 ± 1.0

65.0 ± 2.2

  1. aCalculated from the five independently built ANN classifiers.
  2. bSee Supplementary Notes for terminology.
  3. cMean percentage ± one SD of each individual standard (n = 5000 subsampled cells) in silico mixed to a background of a lake water microbial community (n = 5039), attributed to its own class.