Fig. 1: Glycolate supplementation rescues mitochondrial membrane potential and counteracts oxidative stress of paraquat-treated worms through a glutathione-dependent mechanism.
From: Glycolate combats massive oxidative stress by restoring redox potential in Caenorhabditis elegans
a Mitochondrial membrane potential (ΔΨm-MMP) after 2 days of PQ2+ treatment supplemented or not with glycolate or l-lactate. Panels on the left display mitochondrial fluorescence intensity (scale bar 10 µm) with the corresponding quantification on the right (n = 4). b Effect of inhibition of glutathione synthesis or glutathione supplementation on mitochondrial membrane potential (n = 3). In a and b fluorescence intensities were recorded after MitoTracker Red CMXROS staining. c H2O2 levels in C. elegans worms treated in the same conditions as in (b). After 2 days of treatment, H2O2 accumulation was measured with AmplexRed and fluorescence intensity was normalized to protein levels (n = 4). Error bars represent standard error of the mean. 200 μM paraquat (PQ2+), 10 mM glycolate (GA), 10 mM l-lactate (LL), 0.5 mM BSO, and 1 mM GSH were used as indicated.
