Fig. 8: Glycolate supplementation, via serine–glycine metabolism, increases the ratio of NADPH to NADP+ of peroxide-treated worms.
From: Glycolate combats massive oxidative stress by restoring redox potential in Caenorhabditis elegans
Ratio of NADPH to NADP+ quantified by LC–MS and normalized to protein levels in samples in N2 worms treated as follows. a Animals were exposed to peroxide together with glycolate, glycine, or l-serine, as indicated, for 3 days. b Worms fed with empty vector (EV) or RNAi bacteria against SHMT (mel-32) genes were incubated with H2O2 alone or together with glycolate for 3 days. Error bars represent standard error of the mean (n = 4). 100 mM H2O2, 10 mM glycolate, 100 μM glycine, and 500 μM l-serine.
