Fig. 6: Amylin drives the expression of HIF1α and PFKFB3 in hiPSC-CMs. | Communications Biology

Fig. 6: Amylin drives the expression of HIF1α and PFKFB3 in hiPSC-CMs.

From: Amylin deposition activates HIF1α and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates

Fig. 6

a, b Effect of pre-treatment of hiPSC-CMs with a human amylin antibody for 24 h on amylin-induced HIF1α (a) and PFKFB3 (b) mRNA levels (n = 6/group). c Effect of pre-treatment of hiPSC-CMs with siRNA for HIF1α (siHIF1α) or for vehicle control (siCTL) on amylin-induced HIF1α (n = 4/group). d Effect of pretreatment of hiPSC-CMs with siHIF1α, siRNA for PFKFB3 (siPFKFB3), or siCTL on amylin-induced PFKFB3 (n = 4/group). e, f Effect of HIF1α inhibitor YC-1 on the upregulation of HIF1α (e) and PFKFB3 (f) induced by amylin (n = 6/group). Data represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by One-way ANOVA with Tukey’s post-test.

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