Fig. 4: Interaction between PRPK and TPRKB. | Communications Biology

Fig. 4: Interaction between PRPK and TPRKB.

From: Crystal structure of the human PRPK–TPRKB complex

Fig. 4

a ConSurf style representation of the TPRKB surface involved in PRPK interaction. PRPK is shown as semi-transparent cartoon in white color. b ConSurf style representation of the PRPK surface involved in TPRKB interaction. TPRKB is shown as semi-transparent cartoon in white color. c Detailed interaction between PRPK and TPRKB. PRPK F52, F102, and F103 constitute a hydrophobic surface hosting the TPRKB helix α2. Hydrogen bonds are formed between PRPK Q53 and TPRKB D8, between PRPK R86 and TPRKB V174, and lastly between PRPK V101 and TPRKB S170 (dashed lines). Salt bridges link PRPK R55 with TPRKB D163, and link PRPK D105 with TPRKB H63 (dashed lines). d Pulldown of His-tagged TPRKB by GST-tagged PRPK mutants. GST-tagged PRPK was first immobilized on glutathione Sepharose beads and then incubated with a purified His-tagged TPRKB protein. After extensive washing, His-TPRKB bound to PRPK was detected using a His antibody. A representative result from at least three repetitions is shown. Uncropped images of gel and blot are shown in Supplementary Fig. S6.

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