Table 1 Comparison of kinetic parameters for linalool synthases and LNSs from fungi, plant, and bacteria.
Sesquiterpene synthase | Monoterpene synthase | |||||||
|---|---|---|---|---|---|---|---|---|
Enzymes | Aa.LNS | Zm.LNS | Sc.LNS | Ap.LS | Aa.LNS | La.LS | Ma.LS | Sc.LNS |
Accession no. | / | Q29VN2 | D5SL78 | / | / | Q2XSC5 | Q8H2B4 | D5SL78 |
Product | Nerolidol | Nerolidol | Nerolidol | (R)-linalool | (R)-linalool | (R)-linalool | (R)-linalool | (R)-linalool |
Organism | Agrocybe aegerita | Zea mays | Streptomyces clavuligerus | Agrocybe pediades | Agrocybe aegerita | Lavandula angustifolia | Mentha aquatica | Streptomyces clavuligerus |
Kingdom | Fungi | Plantae | Bacteria | Fungi | Fungi | Plantae | Plantae | Bacteria |
Km (μM) | 9.0 ± 2.3 | 18.0 ± 7.8 | 9.6 ± 0.8 | 3.8 ± 0.7 | 6.7 ± 4.6 | 42.7 | 25 ± 6 | 12.9 ± 1.3 |
kcat (min−1) | 3.3 ± 0.3 | — | 18.7 ± 0.7 | 6.0 ± 0.3 | 0.5 ± 0.1 | 2.34 | 14.4 ± 1.2 | 5.0 ± 0.1 |
kcat/Km (min−1 μM−1) | 0.4 | — | 1.9 | 1.6 | 0.1 | 0.05 | 0.6 | 0.4 |
Reference | This study | This study | This study | |||||
