Fig. 5: CLEC18A and CLEC18A(S339R) enhance TLR3-mediated signaling and IFN production.
From: Endosomal TLR3 co-receptor CLEC18A enhances host immune response to viral infection
a BMDMs (1 × 106/well) from WT and CLEC18 KI mice were preincubated with various concentrations of TLR3/dsRNA complex inhibitor for 1 h, followed by the addition of HMW poly (I:C) (25 μg/ml) for 12 h before harvesting samples to determine cytokine expression by RT-PCR (n = 3). b Alternatively, BMDMs (3 × 104/well) were preincubated with various concentrations of TLR3/dsRNA complex inhibitor for 1 h, followed by the addition of HMW poly (I:C) (25 μg/ml) for 2 h for FRET assay (n = 3–8). c BMDMs (2.5 × 106/well) were incubated with HMW poly (I:C) and then lysates were harvested at indicated time points, followed by harvesting samples to analyze protein phosphorylation by WB. pIC poly (I:C), min minute (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
