Fig. 4: Downregulation of autophosphorylation of RIIβ decreased inhibitory afferent, and RIIβ null increased inhibitory afferent of DG granule cells without changing excitatory afferent in mice.
From: PKA-RIIβ autophosphorylation modulates PKA activity and seizure phenotypes in mice
a Representative raw recording of miniature inhibitory postsynaptic currents (mIPSCs) in DG neurons transfected with AAV-Vector (upper trace, black) and AAV-RIIβ-S112A (lower trace, red). b Comparison of the average values ± SEM of mIPSC frequency. n = 17 neurons for AAV-Vector group and n = 18 neurons for AAV-RIIβ-S112A group, *P < 0.05, unpaired two-tailed Student’s t-test. c Cumulative probability plots of inter-event interval distributions. Insets: comparison of the representative of an averaged mIPSC. d Representative raw recording of miniature excitatory postsynaptic currents (mEPSCs) in DG neurons transfected with AAV-Vector (upper trace, black) and AAV-RIIβ-S112A (lower trace, red). e Comparison of the average values ± SEM of mEPSC frequency. n = 18 neurons for each group, ns no significance, P > 0.05, unpaired two-tailed Student’s t test. f Cumulative probability plots of inter-event interval distributions. Insets: comparison of the representative of an averaged mEPSC. g Representative raw recording of miniature inhibitory postsynaptic currents (mIPSCs) in WT (upper trace, black) and RIIβ−/− (lower trace, blue) DG neurons. h Comparison of the average values ± SEM of mIPSC frequency. n = 15 neurons for WT group and n = 16 neurons for RIIβ−/− group, *P < 0.05, unpaired two-tailed Student’s t test. i Cumulative probability plots of inter-event interval distributions. Insets: comparison of the representative of an averaged mIPSC. j Representative raw recording of miniature excitatory postsynaptic currents (mEPSCs) in DG neurons transfected with AAV-Vector (upper trace, black) and AAV-RIIβ-S112A (lower trace, red). k Comparison of the average values ± SEM of mEPSC frequency. n = 16 neurons for each group, ns no significance, P > 0.05, unpaired two-tailed Student’s t test. l Cumulative probability plots of inter-event interval distributions. Insets: comparison of the representative of an averaged mEPSC. Data were represented as mean ± SEM.
