Fig. 6: Decreased neuronal intrinsic excitabilities of DG granule cells at normal RMP in RIIβ^−/− mice.

a Representative current-clamp recordings of DG neurons held at the normal RMP from WT (black) and RIIβ^−/− (blue) mice. A series of 400-ms hyperpolarizing and depolarizing steps in 50-pA increments were applied to produce the traces. Inset: representative trace in response to 150 pA positive current injection. b Mean number of action potentials (APs) generated in the response of depolarizing current pulses at RMP. n = 17 neurons for WT group and n = 13 neurons for RIIβ^−/− group, **P < 0.01; ***P < 0.001, unpaired two-tailed non-parametric Mann–Whitney U-test for each current pulse. c Representative current-clamp recordings of DG neurons held at a fixed potential of −80 mV from WT (black) and RIIβ^−/− (blue) mice. A series of 400-ms hyperpolarizing and depolarizing steps in 50-pA increments were applied to produce the traces. Inset: representative trace in response to 150 pA positive current injection. d Mean number of APs generated in the response of depolarizing current pulses at −80 mV. n = 18 neurons for WT group and n = 16 neurons for RIIβ^−/− group, **P < 0.01, unpaired two-tailed non-parametric Mann–Whitney U-test for each current pulse. e Individuals and mean spike RMP values. **P < 0.01, unpaired two-tailed Student’s t-test. f Individuals and mean input resistance values at RMP. **P < 0.01, unpaired two-tailed Student’s t-test. g Individuals and mean input resistance values at −80 mV. *P < 0.05, unpaired two-tailed Student’s t-test. h Plot of a typical action potential showed its various phases as the action potential passes a point on a cell membrane. i Typical spikes of DG neurons from WT (black) and RIIβ^−/− (blue) mice at the normal RMP. j Associated phase plane plots. k–m Individuals and mean spike threshold, amplitude, and fAHP values. ns no significance, P > 0.05; *P < 0.05, unpaired two-tailed Student’s t test. Data were represented as mean ± SEM.