Fig. 2: Agonistic effect of menthol, but not camphor, involves R567 in mouse TRPV3.
From: Structural basis for promiscuous action of monoterpenes on TRP channels
a Representative current traces for HEK293T cells expressing wild type (WT) or R567K mutant stimulated with menthol (3 mM) followed by camphor (10 mM). b Comparison of the normalized current densities (Imenthol/Icamphor) at ±60 mV (WT: 0.16 ± 0.03 at +60 mV and 0.08 ± 0.02 at −60 mV, n = 13; R567K: 0.07 ± 0.02 at +60 mV and 0.02 ± 0.01 at −60mV, n = 11). c Comparison of camphor-activated current densities for HEK293T cells expressing WT or mTRPV3-R567K at ±60 mV (WT: 736.5 ± 198.8 pA/pF at +60 mV and 633.5 ± 153.6 pA/pF at −60 mV, n = 6; R567K: 634.9 ± 68.2 pA/pF at +60 mV and 613.6 ± 35.7 pA/pF at −60 mV, n = 9). Holding potentials were −60 mV with ramp-pulses (−100 to +100 mV, 300 ms) every 3 s. Data represent means ± SEM. Statistical analysis was performed by two-sample t-test, *p < 0.05.
