Fig. 5: Positive charge of R567 is essential for camphor-evoked activation of mouse TRPV3.
From: Structural basis for promiscuous action of monoterpenes on TRP channels
a Representative current traces for HEK293T cells expressing WT, mTRPV3-R567A, or mTRPV3-R567F in response to 2-APB (300 μM) treatment followed by camphor (10 mM) stimulation. b Comparison of normalized current densities (Icamphor/I2-APB) in HEK293T cells expressing WT, mTRPV3-R567K, mTRPV3-R567H, mTRPV3-R567A, or mTRPV3-R567F at ±60 mV (WT: 1.93 ± 0.25 at +60 mV and 2.79 ± 0.48 at −60 mV, n = 16; R567K: 1.82 ± 0.22 at +60 mV and 1.95 ± 0.34 at −60 mV, n = 11; R567H: 1.77 ± 0.17 at +60 mV and 1.92 ± 0.28 at −60 mV, n = 12; R567A: 0.21 ± 0.02 at +60 mV and 0.25 ± 0.03 at −60 mV, n = 9; R567F: 0.13 ± 0.01 at −60 mV and 0.18 ± 0.03 at −60 mV, n = 12). Holding potentials were −60 mV with ramp-pulses (−100 to +100 mV, 300 ms) applied every 3 s. Data represent means ± SEM. Statistical analysis was performed by one-way ANOVA, *p < 0.05 (R567A vs. R567K, R567H at −60 mV), **p < 0.01 (R567F vs. R567K, R567H at −60 mV) and ***p < 0.001 (R567A vs. WT, R567K, R567H at +60 mV; R567F vs. WT, R567K, R567H at +60 mV; R567A vs. WT at −60 mV; R567F vs. WT at −60 mV).
