Fig. 5: Expression of the tumour microenvironment-related genes in TGF-β-stimulated cancer cells.
From: Whole-organ analysis of TGF-β-mediated remodelling of the tumour microenvironment by tissue clearing
Identification of targets of TGF-β in A549 cells using RNA-seq analysis. a Expression of epithelial-mesenchymal transition (EMT) markers in A549 cells. A549 cells were cultured in the absence (“Tβ−”) or presence of TGF-β1 (“Tβ+”) for 3 days. Then, gene expression level was determined by RNA-seq analysis. Fragments per kilobase of exon per million reads mapped (FPKM) values of each gene are shown. b–d Gene expression data obtained in a were used for gene set enrichment analysis (GSEA). A list of the most enriched hallmark gene in “Tβ+” sets is presented in b. The normalized enrichment score (NES) and false discovery rate (FDR) are shown. Representative enrichment plots of the hallmarks are shown in c. Heat map showing the expression of genes listed in the hallmarks in d. Genes which were extracted in either or both hallmarks are listed.
