Fig. 4: Effect of in vitro aging during blood storage on the D50 of RBC.

a D50 changes during storage (Day 0: n = 72, Day 1: n = 71, Day 7: n = 60, Day 14: n = 55, Day 28: n = 75 and Day 42: n = 65 of biologically independent RBCs). b–f Probability distribution of D50 for each respective storage day: Day 0 (light blue), Day 1 (red), Day 7 (green), Day 14 (orange), Day 28 (dark blue), and Day 42 (black). Broken lines represent the median values of D50. The significant difference relative to Day 0: *P < 0.05, **P < 0.0001. The significant difference relative to Day 1: †P < 0.001, ††P < 0.0001. The significant difference relative to Day 7: §P < 0.001, §§P < 0.0001. Error bars correspond to the standard deviation of the data.