Fig. 1: Target sequence (QEEYSAM) in RAS and in the active site of a RAS-specific protease.
From: Engineering subtilisin proteases that specifically degrade active RAS
a Structure of RAS with a bound GTP analog (PDB code 6Q21)5,6, highlighting the YSAM site in Switch 2. b RAS-specific protease based on an X-ray structure of 3BGO.pdb17. Cognate sequence QEEYSAM-RD is modeled in the binding cleft. Substrate residues are denoted P1 through P5, numbering from the scissile bond toward the N-terminus of the substrate. The substrate amino acid on the leaving group side is denoted P1’.
