Fig. 1: Susceptibility of PTH4- and/or PTH3-deficient mutants of S. cerevisiae to temperature and antibiotics. | Communications Biology

Fig. 1: Susceptibility of PTH4- and/or PTH3-deficient mutants of S. cerevisiae to temperature and antibiotics.

From: A stalled-ribosome rescue factor Pth3 is required for mitochondrial translation against antibiotics in Saccharomyces cerevisiae

Fig. 1

a The wild type (WT), pth4Δ, pth3Δ, and pth4∆pth3∆ were serially diluted and then spotted onto YPD and YPG plates containing glucose and glycerol as a carbon source, respectively. Plates were incubated at 30 °C or 37 °C for the indicated hours. The results of all of the spotting assays in the present study are shown for one experiment representative of at least three independent experiments. b The four strains were serially diluted and then spotted onto YPG plates containing the following antibiotics, with the concentration shown in parentheses (µg/mL): tetracycline (100), oxytetracycline (200), doxycycline (100), azithromycin (75), erythromycin (20), chloramphenicol (1000), paromomycin (100), tobramycin (100), and streptomycin (1000). Plates were incubated at 30 °C for 48 h (tetracycline, oxytetracycline, and doxycycline) or 72 h (the others). Corresponding images of cells grown on YPD plates for 24 h are shown in Supplementary Fig. 2.

Back to article page