Fig. 1: Relation between the copy number in the genome and degree of MAGGY DNA methylation in Pyricularia oryzae.

a A transposition-deficient MAGGY mutant, MGY-∆RT was introduced into a MAGGY-free P. oryzae strain, Br48 (WT) and a double deletion mutant of Rhm51 and MGG_15577 (∆11350). The integrated copy numbers and the rates of DNA methylation of MGY-∆RT were assessed by qPCR and qMSRE methods with a HapII (CCGG) restriction enzyme, respectively. As a control, pEGFP75 carrying the EGFP gene driven by the Aspergillus nidulans TrpC promoter was introduced into Br48 (WT_GFP). The dashed line represents the regression line in WT_MGY-∆RT. b Southern blot analysis of DNA methylation of MGY-∆RT. Three transformants having different integrated copy numbers of MGY-∆RT were picked up from WT and ∆11350, respectively, and subjected to Southern blot analysis using methylation-insensitive MspI (M) and -sensitive HapII (H) restriction enzymes. *, the integrated copy number of MGY-∆RT.