Fig. 6: Images of plant samples acquired with Pocket MUSE.
a A cluster of filamentous algae stained with 0.05% w/v rhodamine B and 0.01% w/v DAPI. A close-up view of the region labeled with the box (box size: 600 × 600 µm2) is shown on the right. b A cluster of different filamentous algae, (c) a cross-section of a clover stem, (d) a cross-section of a pine needle, (e) a cross-section of onion, and (f), (g) cross-sections of potato stained with the same rhodamine B and DAPI solution. The potato slice in (g) is further washed in saturated iodine water. The stain effectively created a large variety of structural contrast in different plant samples. In general, rhodamine B has a high affinity to xylem structures (c) and (e), while DAPI stains carbohydrate abundant structures such as cell walls (c), and (f), root saps (e), and starch granules (f). In the filamentous algae sample (b), rhodamine B appears to have a higher affinity to some algae cells (appears yellow), while some cells are only stained with DAPI (appears blue). Starch granules are stained in black by elemental iodine, indicating absorptive stains may work collaboratively with fluorescent stains under MUSE contrast. Scale bars: 300 µm.
