Fig. 6: Knockdown of ApoF or β-catenin has similar effects in NSFs.

a Immunoblot confirming downregulation of ApoF upon ApoF and β-catenin knockdown in NSFs. β-tubulin was used as a loading control. b Densitometric quantification of β-catenin and ApoF expression from the immunoblots shown in a. Data are expressed as the mean ± standard deviation (n = 3 biologically independent experiments). *P < 0.05 and ***P < 0.001, two-sided Student’s paired t test. c Immunofluorescence images demonstrating decreased abundance of lipid droplets upon β-catenin or ApoF knockdown (left). Scale bars, 10 μm. Quantitative results showing percentage coverage of cells stained with ORO versus that by total cells in the ORO assay (right). Data are expressed as the mean ± standard deviation (n = 3 biologically independent experiments). ***P < 0.001, two-sided Student’s t test. d Representative images showing SA-β-gal activity in control, β-catenin, and ApoF knockdown NSFs (left). Quantitative analysis of SA-β-Gal-stained cells in control, β-catenin, and ApoF knockdown cells (right). Data are expressed as the mean ± standard deviation (n = 3 biologically independent experiments); n.s., non-significant; **P < 0.01 and ***P < 0.001, two-sided Student’s paired t test.