Fig. 4: Super-affinity IL-1Ra leads to a pro-healing microenvironment.
a Full-thickness wounds were created in Leprdb/db mice and treated with saline control or IL-1Ra variants (0.5 μg of wild type, equimolar IL-1Ra/PlGF123–141). After 6, 9 and 12 days wound concentrations of cytokines, MMPs, TIMP-1 and growth factors were measured by ELISA. The heat map shows fold change in log2 over treatment with saline. n = 4. b Dermal fibroblasts were cultured with IL-1β (1 ng/ml) or saline control. After 9 days, SA-β-gal activity was assessed using senescence green probe (SGP). Graph shows median fluorescence intensity (MFI). n = 6. c Full-thickness wounds in Leprdb/db mice were treated with saline or IL-1Ra variants. After 9 days, SA-β-gal activity in wound fibroblast was assessed by flow cytometry using SGP MFI. SA-β-gal activities were compared to fibroblasts in non-injured skin samples. n = 4. For all panels, data are means ± SEM. For (a), two-way ANOVA with Bonferroni post hoc test for pair-wise comparisons between IL-1Ra and IL-1Ra/PlGF123–141. For (b), two-tailed Student’s t test. For (c), one-way ANOVA with Bonferroni post hoc test for pair-wise comparisons. * P ≤ 0.05, ** P ≤ 0.01, ***P ≤ 0.001. ns non-significant.
