Fig. 6: ATRA and EPI are synergistic in blocking cell cycle progression. | Communications Biology

Fig. 6: ATRA and EPI are synergistic in blocking cell cycle progression.

From: Pin1 inhibition improves the efficacy of ralaniten compounds that bind to the N-terminal domain of androgen receptor

Fig. 6

a Expression of cell cycle proteins from lysates of LN95 cells treated with 0–10 µM of ATRA or 13cisRA. b Graphs show the quantified levels of the indicated proteins after normalizing to β-actin (n = 4). c Cell cycle analysis of LN95 cells treated with vehicle (DMSO), ATRA (5 or 10 μM), EPI-002 (25 μM), or combinations for 24 or 48 h in media supplemented with 1.5% CSS. BrdU-negative S phase cells are denoted by asterisk (*). d Staining of SA β-gal activity performed on LN95 cells after incubating with ATRA (10 μM), EPI-002 (25 μM), ATRA (10 μM), or a combination for 3 days. Original magnification: ×100. The scale bar represents 50 µm. e The graph shows the percentage of β-gal-positive cells scored from at least 1000 cells for each treatment group from three independent experiments. f, g Representative immunoblots showing the expression of indicated cell cycle proteins after incubating with combination therapy. Data shown are the means ± s.e.m. from 3–4 independent experiments. Statistical significance was determined by ANOVA using the Dunnett’s multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001.

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