Fig. 4: En bloc 3D imaging in 25 µm thick tissue slice revealed different Bassoon patterns in three distinct hippocampal circuits. | Communications Biology

Fig. 4: En bloc 3D imaging in 25 µm thick tissue slice revealed different Bassoon patterns in three distinct hippocampal circuits.

From: Targeted volumetric single-molecule localization microscopy of defined presynaptic structures in brain sections

Fig. 4: En bloc 3D imaging in 25 µm thick tissue slice revealed different Bassoon patterns in three distinct hippocampal circuits.The alt text for this image may have been generated using AI.

a Confocal overview of a 25 µm thick section of the hippocampus in a Thy-1 EGFP(M) mouse with imaging windows in the mossy fiber tract (MFT), perforant pathway (PP), and Schaffer collaterals (SC) depicted to scale (25 × 25 µm). 3D plots of Bassoon clusters in MFT (b), PP (c), and SC (d). Cumulative plots of localization counts (e), cluster length (f), and volumes (g) in MFT (blue), PP (magenta), and SC (black). Corresponding data as box plots in h–j Scale bar: 200 µm in a and grid size 2 µm in b–d. Asterisks (***p < 0.001) denote statistical significance MFT vs. PP/SC and PP vs. SC.

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