Fig. 2: Comparison of the metabolic and inflammatory profiles of adipose tissues from WT and Inpp4b^−/− males.

a Protein lysates from eWAT were analyzed for FAS, HK2, CD68, and tubulin by western blot. b Quantification of FAS protein expression in (a). The FAS values were normalized to tubulin and are shown as fold change (N = 3 per group). c–g RNA was extracted from mouse eWAT of LFD WT (N = 12), LFD Inpp4b^−/− (N = 11), HFD WT (N = 8), and HFD Inpp4b^−/− (N = 9) mice. The expression levels of Lep/Adipoq (c), Il6 (d), Tnf (e), Adgre1 (f), and Cd68 (g) were assayed by qRT-PCR using 18S as an internal control. h Quantification of CD68 protein in (a). Average expression in LFD WT mice was designated as 1 (N = 3 per group). i–l Concentrations of leptin (i), insulin (j), C-peptide (k), Resistin (l) in serum of LFD WT (N = 11), LFD Inpp4b^−/− (N = 11), HFD WT (N = 9), and HFD Inpp4b^−/− (N = 9) mice (^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001. Exact p values are provided in Supplementary Data 2).