Fig. 2: Neutrophils and monocytes rapidly migrate to the local skin following subcutaneous injection with S. aureus Lpp. | Communications Biology

Fig. 2: Neutrophils and monocytes rapidly migrate to the local skin following subcutaneous injection with S. aureus Lpp.

From: Staphylococcus aureus lipoproteins promote abscess formation in mice, shielding bacteria from immune killing

Fig. 2

A Representative FACS gating strategy and dot plots of cells isolated from auricular skin tissue following subcutaneous (s.c.) injection of phosphate-buffered saline (PBS) (upper panel) or purified Staphylococcus aureus lipoprotein, denoted as Lpl1 (1 μg) (lower panel) in the mouse ear in C57BL/6 wild-type (WT) mice. Frequencies of infiltrating neutrophils (B), infiltrating monocytes (C), skin macrophages (D), NK cells (E) and T cells (F) in the auricular skin tissue of C57BL/6 WT and TLR2 deficient (TLR2−/−) mice (n = 5–6/group) on day 1 after s.c. injection with 10 μl of PBS or Lpl1 (1 μg/site). The data were pooled from three independent experiments. Statistical evaluations were performed using the Mann–Whitney U test, with data expressed as the median. *P < 0.05; **P < 0.01.

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