Fig. 2: STED microscopy of CD8+ T cell conjugates with APCs and melanoma cells. | Communications Biology

Fig. 2: STED microscopy of CD8+ T cell conjugates with APCs and melanoma cells.

From: Adhering interacting cells to two opposing coverslips allows super-resolution imaging of cell-cell interfaces

Fig. 2: STED microscopy of CD8+ T cell conjugates with APCs and melanoma cells.

a STED images of cell conjugates of CD8+ T cells with T2 cells loaded with the activating peptide NY-ESO-1. The PM of the CD8+ T cells was stained for αCD45 and Alexa647 (red) and the PM of the T2 cells was stained using DPEE-Atto565 (green). Images are shown at different heights (focal planes) relative to the cell interface. b Three-dimensional rendering of the cell conjugates in panel a. c Zoom images of the confocal and STED images are shown for the zoom area in panel a at z = 0 (middle image). Intensity of either green or red channels is shown across line profiles (indicated as colored lines, respectively) in the top-row images. The width of small objects (peaks) of interest are indicated. d STED images of cell conjugates of CD8+ T cells with A375 (melanoma) cells. The PM of the CD8+ T cells was stained for αCD45 and Alexa647 (red) and the PM of the A375 cells and the A375 cells were stained using DPEE-Atto565 (green). Images are shown at different heights (focal planes) relative to the cell interface. e Three-dimensional rendering of the cell conjugates in panel d. f Zoom images of the confocal and STED images are shown for the zoom area in panel d at z = 0 (middle image). Intensity of either green or red channels is shown across line profiles (indicated as colored lines, respectively) in the top-row images. Yellow arrowheads indicate features of interest in the image and on the intensity line profiles. The widths of small objects (peaks) of interest are indicated.

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