Fig. 4: RelAA121E ribosome interaction is analogous to RelA.
From: The RelA hydrolase domain acts as a molecular switch for (p)ppGpp synthesis
RT-mutations (deletion and substitutions) in cDNA reads obtained with CRAC analysis of MG1655 encoding relA::HTF or relAA121E::HTF before and 30 min after isoleucine starvation (here named 0 or 30, respectively). Heatmaps show nucleobase positions with increased error-frequencies caused by RelA•RNA crosslinking. Deletions per million, DPM, or Substitutions per million, SPM, are indicated in red and blue respectively. a RT-mutations in the A-site finger (ASF) of 23 S rRNA (nt 880–900). b Close-up on the ASF (PDB: 5IQR) with positions with significant number of RT-mutations. Deletions or substitutions are shown in red and blue, respectively. ZFD and RRM domains of RelA are coloured in pale orange and pale blue. c RT-mutations in helix 15 (H15) of 16 S rRNA (nt 355–375). d Close-up on H15 (PDB: 5IQR) with crosslinking sites. TGS domain of RelA is displayed in pale green. e RT-mutations in the Sarcin-Ricin Loop (SRL) of 23 S rRNA (nt 2650–2670). f Close-up on SRL (PDB: 5IQR) with crosslinking sites. The pseudo-HD of RelA is shown in blue and the position of mutated alanine 121 is highlighted in salmon. RT-mutations from biological replicates see Supplementary Fig. 4a–n.
