Fig. 1: Clone 1–8 GPR35 KD by shRNA results in reduced morphology change, E-cadherin cleavage, and IL-8 secretion. | Communications Biology

Fig. 1: Clone 1–8 GPR35 KD by shRNA results in reduced morphology change, E-cadherin cleavage, and IL-8 secretion.

From: G-protein coupled receptor 35 (GPR35) regulates the colonic epithelial cell response to enterotoxigenic Bacteroides fragilis

Fig. 1

a GPR35 mRNA expression levels relative to GAPDH. GPR35 expression is significantly reduced compared to wild-type (WT) HT29/c1 cells (N = 8) in the GPR35 shRNA clones 1–8 (N = 5, P < 0.01), 1 (N = 5, P < 0.01), and 2 (N = 5, P < 0.01), but not in clones 3, 4, 5, and 6 (N = 2 each, one-sample T test). Dashed line represents WT GPR35 level. Two points of clone 4 (540 and 947%) outside the y axis. b Morphology changes of HT29/c1 cells were reduced by clone 1–8 (N = 3, P < 0.05), and clone 2 (N = 4, P < 0.05), but not clone 1. Dashed line represents WT toxin score (100%). c IL-8 secretion was significantly reduced in all three GPR35 shRNA clones (clone 1–8, clone 1, and clone 2) (N = 3, P < 0.01) compared to HT29/c1 WT cells (N = 6). d Representative pictures of morphology (top panels) and intact E-cadherin (green, bottom panels). Untreated HT29/c1 WT and GPR35 shRNA clone 1–8 cells are shown in (a) and (c), respectively. After 3 h, BFT changed morphology and cleaved E-cadherin in WT cells (b), but not in the GPR35 shRNA clone 1–8 cells (d).

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