Fig. 4: GPR35 knockout in HT29/c1 cells and mouse organoids results in delayed BFT response. | Communications Biology

Fig. 4: GPR35 knockout in HT29/c1 cells and mouse organoids results in delayed BFT response.

From: G-protein coupled receptor 35 (GPR35) regulates the colonic epithelial cell response to enterotoxigenic Bacteroides fragilis

Fig. 4

a CRISPRcas KO of GPR35 in three confirmed clones 2AH5, 2AA9, and 2BE5 resulted in reduced GPR35 mRNA expression levels for 2AH5 (N = 4, P < 0.05) and 2AA9 (N = 4, P < 0.01) but not for 2BE5 (N = 2). b, c Morphology changes following BFT treatment in CRISPRcas KO were only reduced for clone 2AH5 but not for 2AA9 and 2BE5. d E-cadherin cleavage, determined through western blotting, was reduced to 72% in 2AH5 compared to 94.5% in WT HT29/c1 cells, but was not totally inhibited. A representative western blot and corresponding actin controls are depicted (N = 3). e The fold change of IL-8 secretion in BFT-treated versus nontreated control cells was significantly reduced only for the CRISPRcas KO cell line 2AH5 (N = 2, P < 0.05). f Enteroids derived from the WT and GPR35−/− distal mice colon exposed to BFT show a delayed change in morphology at 3h after treatment with BFT in the GPR35−/− condition.

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