Fig. 6: GPR35-knockout mice show reduced lethality and less severe colitis after ETBF colonization. | Communications Biology

Fig. 6: GPR35-knockout mice show reduced lethality and less severe colitis after ETBF colonization.

From: G-protein coupled receptor 35 (GPR35) regulates the colonic epithelial cell response to enterotoxigenic Bacteroides fragilis

Fig. 6

a Percentage survival of clin/strep pretreated GPR35+/+ (WT) and GPR35−/− mice in a severe colitis experiment 3 days post ETBF infection (P = 0.0186) (N = 6 each). b Percentage survival of cefoxitin-treated GPR35+/+ (WT) (N = 22), GPR35+/− (N = 39), and GPR35−/− (N = 26) mice over 7 days post ETBF colonization (P = 0.0367). c Mouse body weight change in cefoxitin and clin/strep-treated mice. Body weights of GPR35+/+ (WT), GPR35+/−, and GPR35−/− mice were compared 3 days after ETBF colonization to their own baseline (day 0). Sham (N = 6 and N = 4), GPR35+/+ ETBF (N = 9 and N = 7), GPR35+/− ETBF (N = 22 and N = 15), and GPR35+/− ETBF (N = 7 and N = 6) for Clin/Strep and Cefoxitin models, respectively. d) Il17a, Nos2, Il22, Cxcl5, Il6, Ifn-γ, Tnf-α, Mt1, and Mt2 expression in the distal colon of GPR35+/+ and GPR35−/− ETBF-colonized mice compared to their sham controls at 3 days post colonization. Significant differences were observed for Il22, Cxcl5, and Mt2. Individual mice are represented in each dot. e H&E-stained sections of mouse distal colons from clin/strep pretreated GPR35+/+ and GPR35−/− mice at 3 days post ETBF colonization compared to the sham. Mild and severe ETBF colitis histology are shown in GPR35+/+ mice with corresponding GPR35−/− mice.

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