Table 1 Antibody conditions western blot.
Primary antibody | Provider | Dilution | Incubation conditions | Secondary antibody | Dilution | Incubation conditions |
|---|---|---|---|---|---|---|
Mouse-anti-beta-arrestin 1 | BD (610551) | 1:1000 | O/N 4 °C | Goat-anti-mouse | 1:5000 | 1 h RT |
Goat anti-beta-arrestin 2 | Abcam (ab31294) | 1:2000 | O/N 4 °C | Donkey-anti-goat | 1:2000 | 1 h RT |
Rabbit anti-Na+K+ATPase | Thermo Scientific (PA5-17251) | 1:1000 | O/N 4 °C | Goat-anti-rabbit | 1:3000 | 1 h RT |
Mouse-anti-HDAC2 | Cell signaling (3F3, #5113) | 1:100 | O/N 4 °C | Goat-anti-mouse | 1:3000 | 1 h RT |
Mouse-anti-beta-actin | Sigma Aldrich (A5441) | 1:10000 | 2 h RT | Goat-anti-mouse | 1:5000 | 1 h RT |
Rabbit anti-E-cadherin | Gift from James Nelson (Stanford University) | 1:1000 | O/N 4 °C | Goat-anti-rabbit | 1:5000 | 1 h RT |
Rabbit anti-GFP | Abcam ([E385], ab32146) | 1:1000 | O/N 4 °C | Goat-anti-rabbit | 1:5000 | 1 h RT |
Mouse-anti-FLAG M2 | Sigma Aldrich (F3165) | 1:1000 | O/N 4 °C | Goat-anti-mouse | 1:5000 | 1 h RT |