Table 1 Antibody conditions western blot.

From: G-protein coupled receptor 35 (GPR35) regulates the colonic epithelial cell response to enterotoxigenic Bacteroides fragilis

Primary antibody

Provider

Dilution

Incubation conditions

Secondary antibody

Dilution

Incubation conditions

Mouse-anti-beta-arrestin 1

BD (610551)

1:1000

O/N 4 °C

Goat-anti-mouse

1:5000

1 h RT

Goat anti-beta-arrestin 2

Abcam (ab31294)

1:2000

O/N 4 °C

Donkey-anti-goat

1:2000

1 h RT

Rabbit anti-Na+K+ATPase

Thermo Scientific (PA5-17251)

1:1000

O/N 4 °C

Goat-anti-rabbit

1:3000

1 h RT

Mouse-anti-HDAC2

Cell signaling (3F3, #5113)

1:100

O/N 4 °C

Goat-anti-mouse

1:3000

1 h RT

Mouse-anti-beta-actin

Sigma Aldrich (A5441)

1:10000

2 h RT

Goat-anti-mouse

1:5000

1 h RT

Rabbit anti-E-cadherin

Gift from James Nelson (Stanford University)

1:1000

O/N 4 °C

Goat-anti-rabbit

1:5000

1 h RT

Rabbit anti-GFP

Abcam ([E385], ab32146)

1:1000

O/N 4 °C

Goat-anti-rabbit

1:5000

1 h RT

Mouse-anti-FLAG M2

Sigma Aldrich (F3165)

1:1000

O/N 4 °C

Goat-anti-mouse

1:5000

1 h RT