Table 2 Primer combination for SYBR green qPCR.
Gene | Species | Primer forward (5’→3’) | Primer reverse (5’→3’) | Bp | Detector |
|---|---|---|---|---|---|
18S rRNA | Human | TTGACTCAACACGGGAAAC | ACCCACGGAATCGAGAAAGA | 81 | SYBR |
GPR35 a+b | Human | GATCAAGCTGGGCTTCTACG | CAGGCTGATGCTCATGTACC | 265 | SYBR |
GPR35b | Human | ACACCGTGGCAGTGAAGAG | CAGGCTGATGCTCATGTACC | 379 | SYBR |
IL-860 | Human | GTTGTAGTATGCCCCTAAGAG | CTCAGGGCAAACCTGAGTCATC | 407 | SYBR |
GAPDH | Human | TCCTGCACCACCAACTGCTTAG | TGCTTCACCACCTTCTTGATGTC | 341 | SYBR |
