Fig. 5: LCMV-Docile exhibited slow propagation compared to the LCMV-WE strain.
From: Slow viral propagation during initial phase of infection leads to viral persistence in mice
a A schematic of LCMV-S-segment replication is shown. b–g BHK-21 cells were infected with LCMV-WE, LCMV-Docile, or both at the indicted MOI’s. At 24 h and 48 h post-infection, BHK-21 cellular RNA was isolated and b GP RNA, c S-IGR RNA, d NP RNA, e Z RNA, f L-IRG RNA, and g L RNA were assessed by RT-PCR (n = 6). h 24 h post-infection, BHK-21 cellular RNA was analysed by northern blot using GP or NP specific probes (one of n = 4 representative blot was shown, uncropped scan blots in Supplementary Fig. 11). i Ratio of GP mRNA/ribosomal RNA, NP mRNA/ ribosomal RNA and genomic viral RNA/ribosomal RNA were quantified (n = 4). j Virus titers were determined from LCMV infected BMDC supernatant at 24 h and 48 h post-infection (n = 6). k BHK-21 cells were infected with LCMV-WE or LCMV-Docile at MOI 0.5, and, at the indicated timepoints post-infection, monensin was added. Sixteen hours later LCMV infected cells were quantified by anti-LCMV-NP staining (n = 5, gating strategy is shown in Supplementary Fig. 12). (Error bars show SEM, *p < 0.05, **p < 0.01, ***p < 0.001, and ns indicates statistically not significant between the indicated groups).
