Fig. 3: Using nanodisc-ID to profile and quantify pMP binding to lipids.
From: Developing Nanodisc-ID for label-free characterizations of membrane proteins
A Heat map of PL efficiencies of the indicated proteins by TurboID-18A nanodiscs containing the indicated lipids. B Equilibrium titration of TurboID-18A nanodiscs with peripheral proteins. The interactions of syt1 with PI (Kd = 16 nM), SecA with CL (Kd = 106 nM), cpx2 with PI (Kd = 12 µM), and EIIAGlc with CL (Kd = 140 µM) were characterized by PL using TurboID-18A nanodiscs. PL efficiencies of the indicated proteins were then plotted against TurboID-18A nanodisc concentrations. Apparent binding affinities were determined by fitting to a one-site binding equation using GraphPad. Data are shown as mean ± s.d., n = 3 independent experiments.
